A recombinant inbred line population of tomato and its genetic map constructed based on a solanum lycopersicum x s. pimpinellifolium cross

A recombinant inbred line (RIL) population of tomato was developed from a cross between a tomato breeding line (NC 84173) and an accession (LA 0722) of the tomato wild species S. pimpinellifolium. NC 84173 is a horticulturally-superior, multiple-disease resistant inbred line that has been used as a parent in production of several commercial tomato hybrid cultivars. LA 0722 is a self-compatible, inbred accession, which was previously identified as a genetic source for fruit quality, disease resistance, and abiotic stress tolerance. The RIL population is in the F9 generation and consists of 145 lines. A genetic linkage map of the population was developed with 191 molecular markers, including 129 RFLPs and 62 RGAs. The genetic map spans 1505 cM of the 12 tomato chromosomes with an average inter-marker distance of 7.9 cM. The RFLP markers were chosen from the high-density map of tomato, previously developed based on a S. lycopersicum  S. pennellii F2 population. The RGA markers were derived using degenerate oligonucleotide primers designed based on conserved leucine-rich repeat (LRR),