Key result
Inactivated whole-virion vaccines prepared from MRC-5 and Vero cell-adapted EV-D68 strains induced protective immunity against EV-D68 in mice, though some adapted strains were less effective than parental strains.
Why the study?
Enterovirus D68 causes respiratory and neurological diseases, but there are currently no licensed vaccines.
Inactivated whole-virion vaccines derived from MRC-5 and Vero cell-adapted EV-D68 strains successfully induced protective immunity in mice, providing a basis for EV-D68 vaccine production.
Supports EV-D68 vaccine development in mice; human trials needed to confirm immunogenicity and protection.
Enterovirus D68 (EV-D68) is a pathogen that causes respiratory and neurological diseases. Currently, there are no licensed vaccines for EV-D68. Here, we adapted EV-D68 to MRC-5 and Vero cells, which are widely used in vaccine manufacturing, to develop EV-D68 strains applicable for vaccine production. We successfully isolated MRC-5 cell-adapted strains by serial passaging in MRC-5 cells. Although efforts to isolate Vero cell-adapted strains through serial passaging of EV-D68 in Vero cells were unsuccessful, we isolated Vero cell-adapted strains by serial passaging of MRC-5 cell-adapted strains in Vero cells. Inactivated whole-virion vaccines were prepared from vaccine-manufacturing cell-adapted strains and mice were immunized with these vaccines. We found that in some cases, the parental and cell-adapted strains induced similar levels of protective immunity against EV-D68, whereas in other cases, the cell-adapted strains were significantly less effective than the parental strains. These data provide valuable information for EV-D68 vaccine production.
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Senpuku et al. (2025) studied Enterovirus D68. Inactivated whole-virion vaccines from cell-adapted EV-D68 strains vs. PBS or parental strains was evaluated on Viral load in tissues and neutralizing antibody titers. Inactivated whole-virion vaccines prepared from MRC-5 and Vero cell-adapted EV-D68 strains induced protective immunity against EV-D68 in mice, though some adapted strains were less effective than parental strains.
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