A review of in vitro andin vivo culture techniques for the study of pancreatic carcinogenesis

Techniques have been developed for long-term organ explant culture of bovine, hamster, and human pancreatic ducts in enriched medium. The explants were then exposed to chemical carcinogens and studied by morphologic and biochemical techniques. Untreated explants have also been successfully xenotransplanted into athymic “nude” mice. Techniques have also been developed to isolate human and bovine pancreatic ductal cells for cell culture. These as well as techniques developed by other authors are discussed.