Excised root tips from sterile Phaseolus coccineus L. seedlings were incubated in sterile l-tryptophan-3H. Ethanol extracts of the roots were separated by thin-layer chromatography, and the IAA area was eluted and run again in a different solvent system. The presence of labeled IAA was detected using three different solvent pair systems.
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Mitchell et al. (1972) studied this question.