Key result
A CAVD-on-a-chip model using porcine cells developed human-like calcified nodules and calcium phosphate mineralizations over 21 days in response to high shear and endothelial cells.
Why the study?
Calcific aortic valve disease has no targeted therapies, motivating the creation of a physiologically relevant preclinical test-bed of the aortic valve.
The development of a physiologically relevant CAVD-on-a-chip model that replicates human-like calcification could provide a valuable test-bed for preclinical studies of targeted therapies for calcific aortic valve disease.
), and endothelial cell presence on calcification. CAVD chips consisted of a collagen I hydrogel, where porcine aortic valve interstitial cells were embedded within and porcine aortic valve endothelial cells were seeded on top of the matrix for up to 21 days. Here, we show that this CAVD-on-a-chip is the first to develop human-like calcified nodules varying in calcium phosphate mineralization maturity resulting from high shear and endothelial cells, specifically di- and octa-calcium phosphates. Long-term co-culture microfluidic studies confirmed cell viability and calcium phosphate formations throughout 21 days. Given that CAVD has no targeted therapies, the creation of a physiologically relevant test-bed of the aortic valve could lead to advances in preclinical studies.
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Mendoza et al. (2022) studied Calcific aortic valve disease (CAVD). High shear and endothelial cells in a CAVD-on-a-chip model was evaluated on Development of human-like calcified nodules and calcium phosphate mineralizations. A CAVD-on-a-chip model using porcine cells developed human-like calcified nodules and calcium phosphate mineralizations over 21 days in response to high shear and endothelial cells.
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