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The enzyme 6‐phosphogluconolactonase (EC 3.1.1.31) is present at high levels in Zymomonas mobilis cells. A simple procedure for its isolation involving dye‐ligand chromatography and gel filtration has resulted in a 500‐fold purification with high recovery. The purified enzyme is a monomer of 26 kDa, and has a high catalytic efficiency with of 9 × 107 M−1 s−1 at 25° C. Two assay procedures for the enzyme are compared, and a simple method of obtaining a solution of 6‐phosphoglucono‐δ‐lactone relatively free of other metabolites is presented.
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R.K. Scopes (1985) studied this question.
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