Adiantum latifolium Lam., a common fern rich in phytochemicals has been used in traditional medicine. We already reported the larvicidal and antibacterial effects of ethyl acetate fraction of methanol extract of its leaves against the coconut pest Oryctes rhinoceros. Bioactivity guided isolation of compounds from methanol extract of leaves of A. latifolium done by TLC, HPTLC analysis and column chromatography. The structural characterization of the bioactive compounds was done by interpreting their mass spectrum given by HPLC-ESI-Q-TOF-MS and comparison with authentic data. Various classes of phytocompounds viz. terpenoids, phenanthrenoids, phenolics and diones have been identified or tentatively characterized from the bioactive fraction showing a synergistic action. It presents a rapid and proactive method for simultaneous assay of biopotencies and characterization of phytocompounds for the preparation of improved natural pharmaceuticals, antimicrobials or biopesticides.
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Kumar et al. (2019) studied this question.
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