Investigation on 1O2 generation ability of di‐sulfonic phthalocyanine zinc isomers using an HPLC–CL system

An HPLC system combining a chemiluminescence detector was applied to estimate the singlet oxygen ((1)O2) generation ability of di-sulfonic phthalocyanine zinc (ZnPcS2 ) isomers. As photosensitizers, ZnPcS2 produces (1)O2 in air-saturated solutions under photoirradiation. The latter reacts with methyl Cypridina luciferin analogue (MCLA) to initiate chemiluminescence. This photoinduced chemiluminescence (PCL) of MCLA provides an easy method for evaluating the isomers' (1)O2 generation ability during a simultaneous HPLC separation procedure. The cis-isomers and trans-isomers of ZnPcS2 show different (1)O2 generation abilities, which are in accordance with differences in their absorption spectra.