What question did this study set out to answer?

To determine whether hydrogen peroxide, iron, and hydroxyl radicals mediate ischemia/reperfusion-induced granulocyte infiltration in feline intestine and assess whether oxidant reactions generate leukocyte chemoattractants.

February 1, 1990AJP Gastrointestinal and Liver Physiology

Role of oxidants in ischemia/reperfusion-induced granulocyte infiltration

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To determine whether hydrogen peroxide, iron, and hydroxyl radicals mediate ischemia/reperfusion-induced granulocyte infiltration in feline intestine and assess whether oxidant reactions generate leukocyte chemoattractants.
Measured tissue myeloperoxidase activity in feline intestinal mucosa during ischemia/reperfusion and in the dermis during chemotaxis assays.
Assessed in vitro granulocyte migration using a Boyden chamber exposed to feline plasma treated with oxidant-generating systems.
Administered catalase, the iron chelator deferoxamine, and hydroxyl radical scavengers (dimethyl sulfoxide and dimethylthiourea) to evaluate their inhibitory effects.
Catalase, deferoxamine, and dimethylthiourea significantly attenuated mucosal granulocyte infiltration following reperfusion, whereas dimethyl sulfoxide showed no inhibitory effect.
Superoxide-generating systems combined with feline plasma failed to stimulate granulocyte migration in either in vivo or in vitro chemotaxis models.
Hydrogen peroxide in the presence of ferrous iron or hemoglobin significantly elevated the chemotactic activity of feline plasma.

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Implication

Experimental study reveals that hydrogen peroxide and iron promote mucosal granulocyte accumulation during intestinal ischemia/reperfusion, indicating oxidants mediate inflammatory tissue injury.

Cite This Study

Zimmerman et al. (1990) studied this question.

synapsesocial.com/papers/6a9341837aad8b2537cd5cf5 https://doi.org/10.1152/ajpgi.1990.258.2.g185

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Implication

Experimental study reveals that hydrogen peroxide and iron promote mucosal granulocyte accumulation during intestinal ischemia/reperfusion, indicating oxidants mediate inflammatory tissue injury.

Role of oxidants in ischemia/reperfusion-induced granulocyte infiltration

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Discussion

Implication

Cite This Study

Role of oxidants in ischemia/reperfusion-induced granulocyte infiltration

Key Points

Discussion

Implication

Cite This Study

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