The TSH secretion from hemipituitary glands is stimulated in vitro by TRF and this action is blocked by large doses of L-thyroxine. The response to TRF is inhibited by both 2,4-dinitrophenol and oligomycin, indicating that energy is required for TRF action. It is not inhibited by cyclohexamide or ouabain.
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Wilber et al. (1968) studied this question.
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