Abstract γ‐Linolenic acid (Z,Z,Z‐6,9,12‐octadecatrienoic acid), a very important polyunsaturated fatty acid is found in the free fatty acid fraction prepared by the hydrolysis of borage oil. Our aim was to enrich this fraction in γ‐linolenic acid using selective esterification. Candida rugosa lipase was used as catalyst after immobilization on the following ion‐exchange resins: Amberlite IRC50, IRA35, IRA93, and Duolite A7, A368, A568. In every case, immobilization modified the lipae's specificity: palmitic, stearic, oleic, and linoleic acids were preferentially esterified compared to γ‐linolenic acid, thus allowing a γ‐linolenic acid enrichment of 3.0.
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Schmitt‐Rozieres et al. (1999) studied this question.
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