Studies on β-Glucosidase Production in Cultured Tissue ofTrifolium repensL.

Variation observed in the growth and cytology of white clover cells is considered in relation to enzyme production in cultured tissue. Variation also occurred in the production of β-glucosidase by groups of cultured cells derived from the same plant and with identical cultural histories. This variability between cultures grown in a completely defined medium, provided an approach to the investigation of the factors affecting β-glucosidase production in cultured cells. Evidence was obtained from Michaelis constants that two distinct β-glucosidases were produced by cultured tissue.