Low Recovery Rate of Helicobacter Pylori from Positive CLO Test Patients Suffering from Dyspepsia

Method: Gastroscopy and gastric biopsy were performed on 53 patients with dyspepsia from January 2012 to January 2013; all were subjected urease CLO test and culture. The CLO-positive biopsies were cultured using brain-heart infusion agar with added blood (7%), and Skirrow's supplement was used for isolating Helicobacter pylori. Inoculated plates were incubated at 37°C for 7–10 days in a microaerophilic incubation environment and examined for suspected Helicobacter pylori colonies. Helicobacter pylori cultures were confirmed by the positive urease, oxidase and rapid antigen test. Cultures of non-Helicobacter pylori bacteria were identified using few phenotypic tests then confirmed by VITEK 2 automated system.