Human beta-globin messenger RNA. II. nucleotide sequences derived from 125I-labeled globin messenger RNA.

Purified human globin messenger RNA CmRNA) was labeled in vitro with 9 and subjected to digestion with T, and pancreatic RNase. The digests were fractionated in two dimensions and gave characteristic fingerprint patterns. The fingerprints were similar to those obtained with =Plabeled cRNA transcribed from human globin cDNA by RNA polymerase. However, a small number of additional or new oligonucleotides were observed in the ‘7 fingerprints which were either absent or very faint in the fingerprints of the cRNA. Because the lz51 exclusively labels cytidine residues,