6 M guanidinium chloride (Gdm.Cl) (pH 5.2, 25”) suggest that Kunitz soybean trypsin inhibitor (STI) undergoes a slow unfolding which requires over 2 weeks to reach completion. The reduced viscosity increased during this time from an initial value of 3.5 ml/g to a final value of 16 to 17
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Bonnie Strayer Leach (1977) studied this question.