Key result
Virus titers in endothelial cells infected after 24 hours in culture were 10-1000 times higher than in organ cultures or freshly detached cells, with no clear correlation to cytokine production.
In human umbilical cord vein endothelial cells, VSV replication does not clearly correlate with the production of antiviral cytokines like IFN, TNF, and IL-6.
In vitro endothelial models may overestimate VSV replication; leaves open cytokine roles in vivo antiviral defense.
We compared the replication of VSV in human umbilical cord vein organ cultures (OC) with that in endothelial cells detached from the vein and infected immediately or infected after 24 h in culture. In four experiments of five, the virus titers obtained in the endothelial cells infected after 24 h in culture were 10-100 times higher than in the OC and 100-1000 times higher than in the freshly detached endothelial cells. To determine whether production of IFN, TNF, and IL-6 in the various cultures correlated with the data for virus yields, we measured the amounts of these cytokines formed. The OC produced considerably larger amounts of all of these. The endothelial cells produced very little IFN and TNF. Overall, there was no clear correlation between virus production and the amounts of these cytokines formed.
No takes yet. Share an insight, caveat, or question.
Zaczyńska et al. (1995) studied VSV infection in endothelial cells. Endothelial cells infected after 24 h in culture vs. Organ cultures (OC) and freshly detached endothelial cells was evaluated on Virus titers and production of IFN, TNF, and IL-6. Virus titers in endothelial cells infected after 24 hours in culture were 10-1000 times higher than in organ cultures or freshly detached cells, with no clear correlation to cytokine production.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: