Key result
Isolation and characterization of Feline calicivirus strain ZZ202306 revealed typical caliciviral architecture, a peak titer of 10^7.96 TCID50/0.1 mL, and close genetic relation to Chinese strains.
Why the study?
This study investigated a suspected Feline calicivirus outbreak at a veterinary facility in Zhengzhou, Henan Province, China.
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This study investigated a suspected Feline calicivirus (FCV) outbreak at a veterinary facility in Zhengzhou, Henan Province, China. RT-PCR analysis confirmed the FCV presence, with subsequent CRFK cell culture propagation leading to the isolation and characterization of strain ZZ202306. Immunofluorescence and Western blot analyses validated the specificity of monoclonal antibodies targeting the FCV VP1 capsid protein. Transmission electron microscopy revealed non-enveloped virions of ~40 nm in diameter, exhibiting typical caliciviral architecture. Viral replication kinetics demonstrated exponential growth between 6 and 18 h post-inoculation, reaching a peak titer of 107.96 TCID50/0.1 mL. Genomic sequencing coupled with phylogenetic reconstruction of the VP1 gene revealed a close genetic relation to domestic Chinese strains and international variants, while maintaining distinct evolutionary divergence from other calicivirus genera.
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Zhang et al. (2025) studied Feline calicivirus (FCV). Feline calicivirus strain ZZ202306 isolation and characterization was evaluated on Viral replication kinetics and genetic evolution. Isolation and characterization of Feline calicivirus strain ZZ202306 revealed typical caliciviral architecture, a peak titer of 10^7.96 TCID50/0.1 mL, and close genetic relation to Chinese strains.
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