Diffuse, submerged growth of BCG bacilli has been obtained in liquid media containing 0.02 per cent Tween 80 and the soluble fraction of human serum heated under acid conditions (pH 2.5) at 65 degrees C. In the absence of glucose or glycerine,-which had a detrimental effect on viability-these cultures consisted predominantly of cells that were living and that remained viable during prolonged storage at temperatures ranging from 4 to 37 degrees C.
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Dubos et al. (1950) studied this question.
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