SUMMARY: Mycobacteria were grown in rocked ┴ tubes (Monod) in a medium which contained asparagine, glycerol, 1·0% (w/v) Tween 80 and no albumin fraction V. This permitted abundant well-dispeTsed diffuse growth. The amount of growth could be measured photometrically.
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Aoyagi et al. (1959) studied this question.