Key result
A cauterization method allowing collection of isolated myocytes from the basal half and whole tissue from the apical half of rat left ventricles yielded identical myocyte dimensions to controls.
A novel cauterization method allows for the collection of isolated myocytes from the basal half and whole heart tissue from the apical half of the same rat heart without altering myocyte dimensions.
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Enables paired myocyte-tissue analyses from single rat hearts without dimensional change; leaves open applicability to other models or disease states.
A technique for isolation of cardiac myocytes and collection of whole heart tissue from individual hearts of adult rats is described in this study. After excision of the apical half of the left ventricle (LV) and cauterization of the cut edge, aortas were cannulated and high-quality isolated cardiac myocytes were collected after collagenase perfusion of the basal portion. Myocyte dimensions from the basal portion of cauterized and noncauterized hearts from matching rats were identical. Additionally, myocyte dimensions from the basal and apical halves of the LV were compared with the use of whole heart-isolated myocyte preps. No regional differences between basal and apical LV myocyte size were found. Therefore, this cauterization method can be used to collect isolated myocytes from the basal half and whole heart tissue from the apical half, with each half being representative of the other with respect to myocyte dimensions.
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Chen et al. (2007) studied this question. Cauterization method for isolation of cardiac myocytes vs. Noncauterized hearts was evaluated on Myocyte dimensions. A cauterization method allowing collection of isolated myocytes from the basal half and whole tissue from the apical half of rat left ventricles yielded identical myocyte dimensions to controls.
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