Fresh oxalated human plasma and washed human red cells, lysed by the addition of four volumes of 0.025 M-NaHCO3, were used as a source of cholinesterase II (pseudo cholinesterase) and cholinesterase I (true cholinesterase) respectively. The activity of the enzyme was estimated by the Warburg technique The enzyme solution was placed in the main compartment
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Burgen et al. (1951) studied this question.