Summary. Total phospholipide, sphingomyelin, cephalin, and lecithin have been determined in rat brain homogenate and in the various cellular fractions obtained by differential centrifugation in isotonic sucrose. It was found that the concentration of all phospholipide fractions, expressed per mg total nitrogen, was considerably lower in the supernatant fluid than in any of the particle fractions. Sphingomyelin and cephalin were localized mainly in the mitochondria (large granules), whereas the concentration of lecithin was highest in the microsomes (small granules). The phospholipide composition of the nuclei could not be established satisfactorily, since this fraction was too impure. Comparison between brains from normal rats and rats in insulin coma revealed no differences in the phospholipide composition of the various cellular fractions.
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Petersen et al. (1955) studied this question.
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