Key result
Hybridization with excess [3H]cDNA probe accurately quantified proviral DNA, revealing 5-6 copies in uninfected cells and about 15 additional copies in avian myeloblastosis virus-infected myeloblasts.
Population
Uninfected and avian myeloblastosis virus-infected cells (erythrocytes and myeloblasts)
Design
Preclinical
Authors
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Supports cDNA hybridization for proviral quantification in avian models; leaves open clinical translation to human retroviral diagnostics.
The use of excess [3H]cDNA probe is an easy and accurate method to quantify the frequency of proviral DNA sequences in cell DNA and to measure small amounts of viral RNA.
Heilmann et al. (1977) studied Avian myeloblastosis virus infection. Hybridization with [3H]cDNA probe excess was evaluated on Quantification of proviral DNA sequences in cell DNA. Hybridization with excess [3H]cDNA probe accurately quantified proviral DNA, revealing 5-6 copies in uninfected cells and about 15 additional copies in avian myeloblastosis virus-infected myeloblasts.
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