Variations in the formal electrochemical potential (E0) and electron-transfer rates (k0) of the blue copper protein azurin have been directly observed. A new method, fluorescent cyclic voltammetry (FCV), was used to resolve the properties of 100–1000 proteins. On this scale, the presence of large variations in the values of both E0 and k0 could be established and several forms of heterogeneity were differentiated.
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Salverda et al. (2010) studied this question.
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