In a recent paper (1) we described methods tropic eomplex. It is the only one which precipitates at 0.2 saturation. The 0.5 saturated ammonium sulfate for the separation of an hypophyseal fraction hay- (SAS) fraction still contains ICSH but also follicle ing as its specific gonadotropic action stimulation stimulating hormone (FSH), luteinizing hormone (LH), of the interstitial tissue of the ovary and testis; and a larger amount of Antagonist. Most of the growtl b laetogenie and thyrotropie hormones also precipitate we have hence designated this the interstitial cell here. The 0.8 SA8 fraction contains Antagomst, almost stimulating hormone ( ICSH). The best method free of gonadotropie contamination, though sometimes for fractionation of the gonadotropic omplex so t,raees of FSH are present, depending on the volumes as to obtain the I CSH is shown in abbreviated of solvents used in the preceding steps. The flavianie acid supernatant fluid is also brought o 0.5 SAS. Like form in Table I. the preceding 0.5 fraction, it contains a mixture of gonadotropic hormones; after the removal of the 0.5 Method. fraction only FSH is left in the supernatant fluid. This An aqueous alkaline extract of sheep pituitaries is is precipitated by tannic acid. Small amounts of An-
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Evans et al. (1937) studied this question.