Thin layer and gas liquid chromatography, (GLC) were employed as complementary techniques to investigate naturally‐occurring steroids in the unsaponifiable matter of bovine muscle and adipose tissue. Three GLC liquid phases, differing in selective partition properties, were used to effectively identify unknown steroids. The results indicate that cholesterol and minor amounts of desmosterol, Δ7‐cholestenol, lanosterol, dihydrolanosterol, dehydromethostenol, Δ8‐methostenol, Δ7‐methostenol, cholestanol and possibly ergosterol were present in the bovine tissues. The minor steroids, with the exception of cholestanol and ergosterol, are steroid precursors in cholesterol biosynthesis. Common hormonal steroids were not found in the unsaponifiables of the tissues.
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Tu et al. (1969) studied this question.
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