In Vitro Studies of 5,10‐Methylenetetrahydrofolate Reductase: Inhibition by Folate Derivatives, Folate Antagonists, and Monoamine Derivatives

Folate monoglutamates and folate antagonists have an inhibitory action on the activity of 5,10-methylenetetrahydrofolate reductase in rat brain. The type of inhibition was studied for dihydrofolic acid using the Lineweaver--Burk transformation. Some of the monoamine alkaloids, the in vitro products of 5,10-methylenetetrahydrofolate reductase, have either a stimulatory or inhibitory effect on the enzyme activity.