The enzymatic properties of both choloroform-only activated and trypsin–chloroform activated bovine fibrinolysin (plasmin) have been studied and compared with trypsin. These studies accentuate the remarkable similarity between plasmin and trypsin but definitely establish their distinct individuality and the validity of the procedure using trypsin to activate plasminogen to plasmin.
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Edward Ronwin (1956) studied this question.