Cytochalasin‐B: Effects on cytokinesis, glycogen and 3H‐D‐glucose incorporation

Time‐lapse pictures of He La cells grown in a medium with low concentrations of Cytochalasin‐B revealed that during division furrowing proceeds normally until the mid‐body forms. Only then was the centripetal contraction of the furrow blocked. In time the contracted furrow relaxed, resulting in a binucleated cell. Cytochalasin‐B exerted a profound depression on the level of glycogen in the cells and on the incorporation of 3H‐D‐glucose into glycogen, glycoprotein and mucopolysaccharides. These observations suggest that sensitivity to the drug may be due more to interference with glucose uptake and utilization than to disruption of putative contractile microfilaments in the cell cortex.