SUMMARY: Bordetella pertussis grew profusely and retained its normal minute coccobacillary form when grown under 0.4 mm. of fluid lying on a Cohen & Wheeler agar medium + charcoal. Microbes grown in this way were fully antigenic in terms of the mouse protection test. It was easy to obtain harvests of 1.2 x 1011 organisms/ml.
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L. B. Holt (1962) studied this question.
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