Key result
Oleandrin and oleandrigenin cross-react variably with commercial digoxin immunoassays and inhibit Na,K-ATPase with IC50 values of 0.62 and 1.23 micromol/L, respectively.
Why the study?
Do oleandrin and oleandrigenin cross-react with commercial digoxin immunoassays and inhibit Na,K-ATPase activity?
Population
In vitro assays evaluating digoxin immunoassays, Na,K-ATPase catalytic activity, and serum protein binding
Comparison
Oleandrin and oleandrigenin vs Digoxin and ouabain
Design
Preclinical
Authors
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May complicate digoxin assay interpretation in oleander exposure; hypothesis-generating for specific diagnostics.
Do oleandrin and oleandrigenin cross-react with commercial digoxin immunoassays and inhibit Na,K-ATPase activity?
Oleandrin and its metabolite inhibit Na,K-ATPase more potently than digoxin and exhibit highly variable cross-reactivity across different commercial digoxin immunoassays, complicating the diagnosis of oleander poisoning.
Jortani et al. (1996) studied Oleander poisoning. Oleandrin and oleandrigenin vs. Digoxin and ouabain was evaluated on Na,K-ATPase inhibition (IC50) and digoxin immunoassay cross-reactivity. Oleandrin and oleandrigenin cross-react variably with commercial digoxin immunoassays and inhibit Na,K-ATPase with IC50 values of 0.62 and 1.23 micromol/L, respectively.
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