Effects of Ticagrelor on Skeletal Muscle Viability, Asymmetric Dimethylarginine, Malondialdehyde and Glutathione in Ischemia-reperfusion Model

Background and Objective: Although many agents have successful clinical results in experimental studies in order to prevent ischemia-reperfusion (I-R) injury, only a few of them have come into use clinically. In this study, the effects of ticagrelor-a new antiplatelet agent on skeletal muscle viability and blood asymmetric dimethylarginine, malondialdehyde and glutathione levels after experimental ischemia-reperfusion injury of lower limb have been analyzed. Materials and Methods: In this study, 21 male rats have been used. To induce lower limbischemia surgically at the ischemia-reperfusion group (n = 7) and ticagrelor group (n = 7) it clamped Infrarenal Abdominal Aorta (IAA) for 2 h after that were moved the clamp for reperfusion for 2 h. At the end of reperfusion, muscle tissue and blood samples of the rats were taken by sacrificing and excising their soleus muscles. Histopathological search was made in tissue samples and ADMA, MDA, GSH levels were studied in blood and muscle tissue. Results: The ADMA and MDA levels in blood and muscle tissue were more increased on I-R group than control group. This increase was less in ticagrelor administered group. Blood GSH levels were significantly more increased in the I-R group than control group. Compared to I-R group it was significantly decreased in ticagrelor group. Increase of muscle tissue GSH level was more significant in I-R group than in control group. In ticagrelor group muscle tissue GSH level was decreased significantly compared to I-R group. Conclusion: It was observed that ticagrelor reduces oxidant level and injury of skeletal muscle at lower limb ischemia-reper fusion injury. It is observed that ticagrelor is protective against ischemia-reperfusion injury.