Lipid‐protein interactions

Interaction of human serum albumin with phosphatidylserine was studied by turbidimetric methods. It was found that human serum albu‐min will bind phosphatidylserine. Analysis by the law of mass action for multiple equilibria has led to the conclusion that human serum albumin possesses a heterogeneity of binding sites for phos‐phatidylserine. The max number of sites and the respective affinity constants were calculated to be : K1 = 2.0 × 105, n1 = 2; K2 = 1.3 × 103, n2 = 30