An Improved In Vitro Culture Procedure for Embryoids Derived from Isolated Microspores of Rape (Brassica napus L.)

An in vitro culture technique was developed that allowed the effective culturing of embryos derived from isolated microspores of rape (Brassica napus L.). The method consists of the use of microspores from buds at well‐defined development stages, the use of a double‐layer culture medium containing activated charcoal and the incubation of embryoids in culture flasks on a shaker. These culture modifications, together with the growing of embryoids on solid medium with high agar concentration, resulted in a high frequency of embryoids growing directly into plants.