BACKGROUND: To develop and validate a rapid, sensitive and selective liquid chromatography-electrospray ionization mass spectrometric method for the determination of meloxicam and its metabolite 5-carboxymeloxicam in human plasma. RESULTS: A liquid extraction method was chosen for sample clean-up. Meloxicam, 5-carboxymeloxicam and isoxicam (internal standard) were analyzed on an XBridge™ C18 column with 65% methanol in 10 mM ammonium formate (pH 3.0) and detected in selected reaction monitoring mode. The standard curves were linear over the concentration range 10-2500 ng/ml for meloxicam and 2-100 ng/ml for 5-carboxymeloxicam. Matrix effects were practically absent. CONCLUSIONS: This method has been successfully applied to the pharmacokinetic study of meloxicam and 5-carboxymeloxicam after oral administration of meloxicam (15 mg) to 30 volunteers.
No takes yet. Share an insight, caveat, or question.
Lee et al. (2009) studied this question.
Synapse has enriched one closely related paper. Consider it for comparative context: