What question did this study set out to answer?

To elucidate the molecular mechanism by which the GTPase Rab13 regulates the assembly of functional epithelial tight junctions.

April 19, 2004The Journal of Cell BiologyOpen Access

Rab13 regulates PKA signaling during tight junction assembly

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Authors

KKKatja KöhlerETH Zurich DLDaniel LouvardUniversity of Geneva AZAhmed ZahraouiInserm

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Implication

Experimental study demonstrates that active Rab13 directly inhibits PKA to modulate tight junction assembly in epithelial cells, highlighting a novel GTPase-kinase signaling link.

Key Points

To elucidate the molecular mechanism by which the GTPase Rab13 regulates the assembly of functional epithelial tight junctions.
Expressed GTP-bound Rab13 to evaluate its effects on PKA-dependent phosphorylation and tight junction recruitment of vasodilator-stimulated phosphoprotein.
Assessed physical binding interactions between Rab13GTP and PKA, and measured PKA enzymatic activity upon binding.
Examined the recruitment of junctional proteins, including ZO-1 and claudin1, following pharmacological activation of PKA in the presence of active Rab13.
Active GTP-bound Rab13 directly binds to PKA and inhibits its kinase activity.
Rab13GTP expression inhibits PKA-dependent phosphorylation and junctional recruitment of vasodilator-stimulated phosphoprotein.
Direct activation of PKA abrogates Rab13-mediated inhibition, restoring the recruitment of vasodilator-stimulated phosphoprotein, ZO-1, and claudin1 to cell-cell junctions.

Cite This Study

Köhler et al. (2004) studied this question.

synapsesocial.com/papers/6a93db105e7ae87f038f927c https://doi.org/10.1083/jcb.200312118

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Implication

Experimental study demonstrates that active Rab13 directly inhibits PKA to modulate tight junction assembly in epithelial cells, highlighting a novel GTPase-kinase signaling link.

Rab13 regulates PKA signaling during tight junction assembly

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Implication

Key Points

Cite This Study

Rab13 regulates PKA signaling during tight junction assembly

Discussion

Member takes

Implication

Key Points

Cite This Study

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