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April 1, 1992Cardiovascular Research

Insulin treatment for 7 days or longer normalized the significant decreases in mitochondrial calcium uptake, transmembrane potential, and cell respiration observed in diabetic rat cardiomyocytes.

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Why the study?

Does insulin treatment improve mitochondrial function in cardiomyocytes of rats with experimental diabetes?

Population

31 male Sprague-Dawley rats, including 20 with streptozotocin-induced diabetes and 11 non-diabetic controls

Comparison

Subcutaneous ultra lente insulin for 7-15 days vs Untreated diabetic rats and non-diabetic controls

Design

Preclinical

Follow-up

21-35 days after induction of diabetes

Key result

Insulin treatment for 7 days or longer normalized the significant decreases in mitochondrial calcium uptake, transmembrane potential, and cell respiration observed in diabetic rat cardiomyocytes.

Authors

YTYuzuru TANAKANKNoburu KonnoKKK.J. Kako

Discussion

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Member takes

Overview

Should not yet inform clinical care in diabetic cardiomyopathy; leaves open insulin's mitochondrial effects as a preventive mechanism in humans.

Structured PICO

Does insulin treatment improve mitochondrial function in cardiomyocytes of rats with experimental diabetes?

P
Population
31 male Sprague-Dawley rats with experimental diabetes or control, evaluating the effects of insulin treatment on mitochondrial function in isolated cardiomyocytes.
I
Intervention
Subcutaneous ultra lente insulin (starting dose 3 units/day) for 7-15 days
C
Comparator
Untreated diabetic rats and non-diabetic controls
O
Outcome
Mitochondrial function in situ in isolated cardiomyocytes (including 45[Ca]2+ uptake, transmembrane potential gradient, and cell respiration)surrogate

Insulin treatment reverses early mitochondrial dysfunction in cardiomyocytes of rats with experimental diabetes, suggesting a potential mechanism for preventing diabetic cardiomyopathy.

Cite This Study

TANAKA et al. (1992) studied Experimental diabetes (n=31). Insulin treatment vs. Untreated experimental diabetes and healthy controls was evaluated on Mitochondrial uptake of [45Ca]2+, transmembrane potential gradient, and cell respiration. Insulin treatment for 7 days or longer normalized the significant decreases in mitochondrial calcium uptake, transmembrane potential, and cell respiration observed in diabetic rat cardiomyocytes.

synapsesocial.com/papers/6a93e4a878fe3e71bd43dad4https://doi.org/10.1093/cvr/26.4.409
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