Bacteria beneath 58 class V Concise restorations on permanent molars in vivo were registered. After an observation period of 1-4 months bacteria were found in all 13 cavities filled with Concise only, in 14 out of 15 cavities filled with resin and Concise, in 12 out of 15 acid-etched cavities with Concise only and in 8 out of 15 acid-etched cavities filled with resin and Concise. Only the difference between the two procedures: Concise only and acid + resin + Concise, was significant. The occurrence of bacteria in the dentinal tubules beneath the cavities was greatest when the cavities had been etched.
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Qvist et al. (1977) studied this question.
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