lating species referred to as Aerobacter levanicum (III) has been studied.All three produced levan from sucrose and raffinose.In I, levansucrase is formed.adaptively; in III it is constitutive.In I, growing on sucrose agar, levansucrase is exocellular and diffuses through the medium; in II and III on the same medium, it is endocellular.3. Levansucrase has been prepared cell-free from III as an aqueous solution, as well as in soluble dry form.The cell-free enzyme systems act on sucrose with rapid formation of levan and of reducing sugar.Certain physical properties of cell-free levansucrase from I and III have been studied.4. Levan has been prepared in substantial amount as a nitrogen-free powder by the action of sterile levansucrase from III on sucrose.Some properties of the enzymically formed polysac- charide are described.The authors are indebted to Dr J. Leibowitz for his continued interest in the progress of this investigation.
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Kodíček et al. (1943) studied this question.