What does this research mean for the field?

A recombinant thermotolerant alkaline lipase from Lysinibacillus fusiformis exhibits high catalytic efficiency, thermostability, and alkaline tolerance, effectively improving detergent oil-stain removal and accelerating Ras cheese ripening. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

To isolate, clone, and express lipase genes from Lysinibacillus fusiformis to produce a thermostable, alkaline lipase for industrial detergent and food applications.

August 30, 2026Microbial Cell FactoriesOpen Access

Recombinant thermotolerant alkaline lipase from Lysinibacillus fusiformis for detergent and hard (Ras) cheese applications: cloning, expression, molecular docking, and characterization

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Authors

GEGhada M. El-SayedCairo University HWHala R. WehaidyNational Research Centre AKA. KholifNorth Carolina Agricultural and Technical State University

Discussion

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Implication

Biochemical study demonstrates high stability and activity of recombinant Lysinibacillus fusiformis lipase, indicating its industrial potential in detergents and accelerated cheese ripening.

Key Points

To isolate, clone, and express lipase genes from Lysinibacillus fusiformis to produce a thermostable, alkaline lipase for industrial detergent and food applications.
Screened bacterial isolates, sequenced the 16S rRNA gene of Lysinibacillus fusiformis (GenBank PP757498), and cloned three lipase genes (est, est2, lipA) into Escherichia coli BL21 (DE3).
Characterized enzyme activity across temperature and pH ranges, performed molecular docking against olive oil fatty acids, and tested applications in fabric stain removal and Ras cheese maturation.
Recombinant lipA lipase (rLipase) demonstrated higher activity than the native enzyme (150 U/mL vs 56.2 U/mL), reaching a maximum activity of 320 U/mL at 80 °C and pH 9.
Molecular docking identified strong binding affinity for linoleic acid (−8.0 kcal/mol), oleic acid (−7.8 kcal/mol), and palmitic acid (−7.3 kcal/mol), stabilized by hydrophobic bonds and residue VAL250.
Supplementation with 0.4% rLipase enhanced oil stain removal from cotton fabric and shortened Ras cheese ripening from 120 to 90 days without compromising quality.

Cite This Study

El-Sayed et al. (2026) studied this question.

synapsesocial.com/papers/6a93f1396c1a8fb52e79dfe9 https://doi.org/10.1186/s12934-026-03084-w

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August 30, 2026Microbial Cell FactoriesOpen Access

Recombinant thermotolerant alkaline lipase from Lysinibacillus fusiformis for detergent and hard (Ras) cheese applications: cloning, expression, molecular docking, and characterization

View Full Paper

Ask AI

Bookmark

Authors

GEGhada M. El-SayedCairo University HWHala R. WehaidyNational Research Centre AKA. KholifNorth Carolina Agricultural and Technical State University

Discussion

Member takes

Implication

Biochemical study demonstrates high stability and activity of recombinant Lysinibacillus fusiformis lipase, indicating its industrial potential in detergents and accelerated cheese ripening.

Key Points

To isolate, clone, and express lipase genes from Lysinibacillus fusiformis to produce a thermostable, alkaline lipase for industrial detergent and food applications.
Screened bacterial isolates, sequenced the 16S rRNA gene of Lysinibacillus fusiformis (GenBank PP757498), and cloned three lipase genes (est, est2, lipA) into Escherichia coli BL21 (DE3).
Characterized enzyme activity across temperature and pH ranges, performed molecular docking against olive oil fatty acids, and tested applications in fabric stain removal and Ras cheese maturation.
Recombinant lipA lipase (rLipase) demonstrated higher activity than the native enzyme (150 U/mL vs 56.2 U/mL), reaching a maximum activity of 320 U/mL at 80 °C and pH 9.
Molecular docking identified strong binding affinity for linoleic acid (−8.0 kcal/mol), oleic acid (−7.8 kcal/mol), and palmitic acid (−7.3 kcal/mol), stabilized by hydrophobic bonds and residue VAL250.
Supplementation with 0.4% rLipase enhanced oil stain removal from cotton fabric and shortened Ras cheese ripening from 120 to 90 days without compromising quality.

Cite This Study

El-Sayed et al. (2026) studied this question.

synapsesocial.com/papers/6a93f1396c1a8fb52e79dfe9 https://doi.org/10.1186/s12934-026-03084-w

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