During the course of an investigation into the bacteriology of the water supplies of the colony the repeated isolation of B. colt (lactose +, indol +) and of Houston's atypical B. colt from small quantities (I c.c., -I c.c., and -oI c.c.) of raw natural waters which were declared on sanitary survey to be free from all possibility of human faecal pollution and very remotely, if at all, liable to any animal contamination, centred attention on the sufficiency of the methods for the bacterioscopic examination of waters recommended by a Committee of the Royal Institute of Public Health in 1903, and adopted by bacteriologists in England and elsewhere as the standard method, and the interpretation of the results obtained by this method.The literature of the bacteriology of tropical waters reveals 'frequent references to the presence of B. colt as detected by the Pawan, J L. 1925."A New Medium for the Differentiation of B.
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J. L. Pawan (1925) studied this question.
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