A new cDNA and two genomic genes encoding the rice storage protein glutelin were isolated and sequenced. The nucleotide sequence of one gene (GluA-3) was completely identical with that of the new cDNA identified here, and the other (GluA-4) was a pseudogene. These glutelin genes were closely related to each other, and belonged to the subfamily A containing the type I (GluA-1) and II (GluA-2) glutelin genes. The Northern blot analysis, using synthetic oligonucleotide specific to the GluA-3 gene as a probe, showed that this gene was expressed earlier than other glutelin genes during seed maturation.
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Takaiwa et al. (1991) studied this question.
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