HPTLC Standardization of an Ayurvedic Polyherbal Tablet for Covid-19 Management

The present study was aimed to scientifically validate the ayurvedic polyherbal tablet formulation used for the treatment of COVID-19. Organoleptic evaluation, physiochemical evaluation such as water-soluble extractive value, alcohol soluble extractive value, pH determination, weight variation, disintegration test, phytochemical investigation and HPTLC analysis, determination of microbial contamination and heavy metal analysis of the Ayurvedic Polyherbal Tablet Formulation were studied. The HPTLC analysis of Ayurvedic Polyherbal Tablet Formulation confirmed 0.04% of rutin in each methanol and aqueous extracts, 0.46% and 0.14% of quercetin, 0.04% and 0.17% of gallic acid, 0.19% of piperine in each methanol and aqueous extracts, 0.11 and 0.32% of andrographolide in methanol and aqueous extracts respectively. Except weight variation, remaining evaluation tests such as extractive value, disintegration time, pH and the phytochemical investigation of Ayurvedic Polyherbal Tablet Formulation were found to be within the pharmacopeial limits. HPTLC investigations of methanol and aqueous extracts of the Ayurvedic Polyherbal Tablet Formulation confirmed the presence of all ingredients mentioned in the Label claim. Microbial tests and heavy metal analysis revealed that the formulation is safe as it does not contain any microorganisms or heavy metals.