An agar medium (Alsan), was selective for Lactococcus lactis subsp. lactis and Lactococcus lactis subsp. lactis biovar. diacetylactis. The former developed white or yellow colonies; the latter gave blue‐green or blue colonies. Eleven strains of L. lactis subsp. cremoris did not grow in this medium. The medium contained phenylethanol agar, lactose, glycine anhydride, lithium chloride and trimethoprim. In comparison with M17 medium, Alsan was not significantly different (P > 0.05) in recovering either bacterium. Furthermore, the medium inhibited the common food genera Pseudomonas, Escherichia, Leuconostoc, Enterococcus and Lactobacillus. Bacteriophage plaque sizes of 0.4–0.5 cm were demonstrated on the medium using a lactococcal bacteriophage.
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Al-zoreky et al. (1991) studied this question.
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