Some factors controlling heteroblastic development in Centaurea solstitialis L. are reported and discussed. Whole plants were grown in sterile culture, and it was demonstrated that the level of available carbohydrates partly accounts for the heteroblastic series. Growing plants in sterile culture supplemented with gibberellic acid prevented the development of the adult leaf form, whereas Centaurea plants grown in media supplemented with Cycocel (CCC) formed adult leaves earlier than plants growing in media lacking CCC. When transferred to a GA-supplemented medium, plants already producing the adult leaf undergo transition to flowering, and all new leaves develop as the juvenile form. It is proposed that the heteroblastic series is in part regulated by the endogenous levels of gibberellins.
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Feldman et al. (1970) studied this question.