Why the study?
Does the choice of anticoagulant and centrifugation method affect the levels of TGFB-1 and PDGF-BB in platelet-rich plasma from healthy males?
Does the choice of anticoagulant and centrifugation method affect the levels of TGFB-1 and PDGF-BB in platelet-rich plasma from healthy males?
Single spin centrifugation and the use of lithium heparin as an anticoagulant yield higher levels of TGFB-1 and PDGF-BB in platelet-rich plasma compared to double spin and other anticoagulants.
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May influence PRP growth factor delivery in regenerative applications; leaves open optimal protocols pending clinical validation.
Background: Platelet-rich plasma (PRP) are platelets concentrates made of autogenous blood with a high number of platelets in a small volume of plasma. The main component that appears to be associated with the therapeutic effect in PRP is the presence of growth factors (GF). Variable techniques for PRP preparation produce variable amounts of growth factors.
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Dr Hoda Elhady Ahmed Abdelmoneim (2018) studied Healthy volunteers (n=30). Lithium heparin and single spin centrifugation vs. EDTA, trisodium citrate, and double spin centrifugation was evaluated on Transforming growth factor beta-1 (TGFB-1) levels in platelet-rich plasma (ng/ml) (p=<0.01).
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