In this paper, a concept of polarimetric total internal reflection (TIR) biosensor based on the method of temporal phase modulation is presented. Measurements of the phase difference between s- and p- polarized light combined with their amplitudes allow simultaneous detection of the bulk refractive index and thickness of the surface biofilms. Obtained experimental sensitivity is better than 10(-5) in terms of refractive index unit and 0.5 nm in biolayer thickness. Relatively simple technological implementation of the TIR sensors on the base of inexpensive and transparent substrates opens a number of novel applications in biosensing and microscopy.
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Maisonneuve et al. (2011) studied this question.
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