Comparison of Dermatophagoides pteronyssinus allergens from culture medium extract and whole body extract by using the same probe of pooled human serum

Two distinct allergens of Dermatophagoides pteronyssinus were isolated from culture medium extract and whole body extract monitored by the same probe of pooled human serum and compared. IgE-binding activity of allergens from the two sources was detected by microplate-ELISA with the same probe throughout the column chromatography. An allergen obtained from culture medium extract (Ag-CME) had a molecular weight of 27,000 by SDS-polyacrylamide gel electrophoresis (PAGE) and two isoelectric point ranges, pI 4.0-4.7 and 5.9-6.8. Another allergen from whole body extract (Ag-WBE) had a molecular weight of 15,000 by SDS-PAGE and an isoelectric point range, pI 4.8-5.3 Ag-CME and Ag-WBE are probably the same allergens as antigen P1 and DpX, respectively.