Protein recognition by bivalent, ‘turn-on’ fluorescent molecular probes

The selective and sensitive identification of different proteins becomes possible by modifying the known intercalating dye, thiazole orange, with two protein binders. These ‘turn-on’ fluorescence probes enable the identification of acetylcholinesterase, glutathione- s -transferases and avidin with high affinity, specificity, and high signal-to-noise ratio.