Environment‐Sensitive Fluorescent Turn‐On Probes Targeting Hydrophobic Ligand‐Binding Domains for Selective Protein Detection

Bind and shine: An approach for the selective detection of both enzymes and non-enzymatic proteins using an environment-sensitive fluorescent turn-on probe is described (see scheme). This approach targets the hydrophobic ligand-binding domain of the target protein to trigger the fluorescence turn-on and was shown to be specific for the targeted protein.